Expanding substrate specificity of nucleoside 2'-deoxyribosyltransferase.

Pierre-Alexandre Kaminski (1) , Laurence Dugué (1) , Sylvie Pochet (1)

1 Chimie Organique

Nucleoside 2'-deoxyribosyltransferase (NDT) is used to synthesize unnatural 2'-deoxyribonucleosides, modified mostly on the heterocyclic base. Here we describe a strategy for improving 2,3-dideoxyribosyl(ddR) transfer activity of NDT by combining mutagenesis and in vivo selection in E. coli.

Domaines: Biochimie, Biologie Moléculaire; Biotechnologies

Pierre-Alexandre Kaminski, Laurence Dugué, Sylvie Pochet. Expanding substrate specificity of nucleoside 2'-deoxyribosyltransferase.. Nucleic Acids Symposium Series, 2008, 52, pp.495-6. ⟨10.1093/nass/nrn251⟩. ⟨pasteur-00325467⟩