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Journal articles
Looking for new pyrimidine acyclic nucleotide analogues designed for phosphorylation by human ump-cmp kinase.
Abstract : Human UMP-CMP kinase is involved in the phosphorylation of nucleic acid precursors and also in the activation of antiviral analogues including cidofovir, an acyclic phosphonate compound that mimicks dCMP and shows a broad antiviral spectrum. The binding of ligands to the enzyme was here investigated using a fluorescent probe and a competitive titration assay. At the acceptor site, the enzyme was found to accommodate any base, purine and pyrimidine, including thymidine. A method for screening analogues based on their affinity for the UMP binding site was developed. The affinities of uracil vinylphosphonate derivatives modified in the 5 position were found similar to (d)UMP and (d)CMP and improved when compared to cidofovir.
https://hal-pasteur.archives-ouvertes.fr/pasteur-00202298
Contributor : Sylvie Pochet Connect in order to contact the contributor
Submitted on : Friday, January 4, 2008 - 6:28:36 PM
Last modification on : Thursday, December 16, 2021 - 3:56:46 AM
Contributor : Sylvie Pochet Connect in order to contact the contributor
Submitted on : Friday, January 4, 2008 - 6:28:36 PM
Last modification on : Thursday, December 16, 2021 - 3:56:46 AM