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Direct isolation of satellite cells for skeletal muscle regeneration.

Abstract : Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.
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Contributor : Odile Delpech Connect in order to contact the contributor
Submitted on : Tuesday, October 23, 2007 - 3:20:24 PM
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Didier Montarras, Jennifer Morgan, Charlotte Collins, Frédéric Relaix, Stéphane Zaffran, et al.. Direct isolation of satellite cells for skeletal muscle regeneration.. Science, American Association for the Advancement of Science (AAAS), 2005, 309 (5743), pp.2064-7. ⟨10.1126/science.1114758⟩. ⟨pasteur-00181349⟩



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