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Direct isolation of satellite cells for skeletal muscle regeneration.

Abstract : Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.
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Contributor : Odile Delpech Connect in order to contact the contributor
Submitted on : Tuesday, October 23, 2007 - 3:20:24 PM
Last modification on : Monday, May 9, 2022 - 11:58:07 AM




Didier Montarras, Jennifer Morgan, Charlotte Collins, Frédéric Relaix, Stéphane Zaffran, et al.. Direct isolation of satellite cells for skeletal muscle regeneration.. Science, 2005, 309 (5743), pp.2064-7. ⟨10.1126/science.1114758⟩. ⟨pasteur-00181349⟩



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