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The Th1 immune response against HIV-1 Gag p24-derived peptides in mice expressing HLA-A02.01 and HLA-DR1.

Abstract : Using HLA-DR1-transgenic H-2 class II knockout mice, we identified two new HLA-DR1-restricted HIV-1 Gag p24-derived epitopes (Gag(321-340 )and Gag(331-350)) and confirmed the immunogenicity of seven that have been previously described. The human relevance was confirmed for the two new ones (Gag(321-340 )and Gag(331-350)) assaying peripheral blood mononuclear cells from HLA-DR1(+) HIV-1-infected long-term asymptomatic subjects and showing that Gag(331-350) could prime CD4(+) T cells from two HLA-DR1(+) HIV-1 seronegative donors in vitro. Seven of these epitopes, structurally conserved among HIV-1 clade B isolates, were selected for a comparative evaluation of their Th1 helper potential by immunizing HLA-A02.01/HLA-DR1-transgenic, H-2 class I/class II knockout mice with recombinant mouse invariant chain constructs in which each helper epitope was inserted in association with two reporter HIV-1-derived HLA-A02.01-restricted CD8(+) T cell epitopes. A T helper effect was demonstrated in all cases, and was particularly strong with epitopes Gag(301-320),( )Gag(321-340 )and Gag(271-290), which should, therefore, be considered in the design of new vaccines.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-00167809
Contributor : François Lemonnier <>
Submitted on : Thursday, August 23, 2007 - 10:10:59 AM
Last modification on : Wednesday, September 16, 2020 - 4:54:57 PM

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Anthony Pajot, Aurélie Schnuriger, Arnaud Moris, Audrey Rodallec, David M Ojcius, et al.. The Th1 immune response against HIV-1 Gag p24-derived peptides in mice expressing HLA-A02.01 and HLA-DR1.. European Journal of Immunology, Wiley-VCH Verlag, 2007, 37 (9), pp.2635-2644 ⟨10.1002/eji.200636819⟩. ⟨pasteur-00167809⟩

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