Crystallization and preliminary X-ray analysis of the thymidylate kinase from Mycobacterium tuberculosis.

Inès Li de La Sierra (1) , Hélène Munier-Lehmann (2) , Anne-Marie Gilles (2) , Octavian Bârzu (2) , Marc Delarue (1)

1 Biochimie Structurale
2 CSM - Chimie Structurale des Macromolécules

Mycobacterium tuberculosis thymidylate kinase complexed with the substrate deoxythymidine monophosphate was crystallized in the hexagonal space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 76.62, c = 134.38 A and one single monomer of 23 kDa in the asymmetric unit. Cryo-cooled crystals diffract at 1.94 A resolution using synchrotron radiation.

Domaines: Sciences du Vivant [q-bio]

Inès Li de La Sierra, Hélène Munier-Lehmann, Anne-Marie Gilles, Octavian Bârzu, Marc Delarue. Crystallization and preliminary X-ray analysis of the thymidylate kinase from Mycobacterium tuberculosis.. Acta crystallographica Section D : Structural biology [1993-..], 2000, 56 (Pt 2), pp.226-8. ⟨10.1107/S0907444999016212⟩. ⟨pasteur-00167073⟩