Predominant intracellular expression of CXCR4 and CCR5 in purified primary trophoblast cells from first trimester and term human placentae.

Juan Maldonado-Estrada (1, 2) , Elisabeth Menu (1, 3) , Pierre Roques (4) , Bruno Vaslin (4) , Alice Dautry-Varsat (5) , Françoise Barré-Sinoussi (3) , Gérard Chaouat (1)

1 Cytokines et immunorégulation
2 Grupo de Reproducción Animal
3 Biologie des Rétrovirus
4 UMR E01 - Laboratoire de Neuro-Immuno-Virologie, Service de Neurovirologie EMI E-01
5 Biologie des Interactions Cellulaires

PROBLEM: The aim of the present study was to define the expression of CXCR4 and CCR5 on non-cultured non-stimulated primary human trophoblast cells (TCs) immediately after their immunopurification. METHOD OF STUDY: We have evaluated by flow cytometric analysis and immunofluorescence, highly purified primary TCs prepared from first trimester (8.2 +/- 0.3 weeks, n = 15) and term (Caesarean section, n = 10) placentae for the cell surface and intracellular expression of CXCR4 and CCR5. RESULTS: There was a high level of individual variability for CXCR4 and CCR5 expression between trophoblast batches. In first trimester and term placentae TCs, we found a greater number of TCs preparations expressing intracellular CXCR4 than CCR5 (P < 0.05). Both receptors were predominantly localized in the intracellular compartment of TCs, whatever if isolated from first trimester or term placentae. CONCLUSIONS: The functional consequences of the predominance of CXCR4 expression and of cellular addressing are briefly discussed.

Domains: Molecular biology

Juan Maldonado-Estrada, Elisabeth Menu, Pierre Roques, Bruno Vaslin, Alice Dautry-Varsat, et al.. Predominant intracellular expression of CXCR4 and CCR5 in purified primary trophoblast cells from first trimester and term human placentae.. American Journal of Reproductive Immunology, 2003, 50 (4), pp.291-301. ⟨10.1034/j.1600-0897.2003.00084.x⟩. ⟨pasteur-00166956⟩