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Deciphering the role of individual acyl chains in the interaction network between phosphatidylserines and a single-spanning membrane protein.

Abstract : PMP1 is a small single-spanning membrane protein functioning as a regulatory subunit of the yeast plasma membrane H(+)-ATPase. This protein forms a unique helix and exhibits a positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines (PSs). A marked groove formed at the helix surface is thought to play a major role in the related lipid-protein interaction network. Mutational analysis and (1)H NMR experiments were therefore performed on a synthetic PMP1 fragment using DPC-d(38) micelles as a membrane-like environment, in the presence of small amounts of POPS. A mutation designed for altering the helix groove was shown to disfavor the POPS binding specificity as much as that affecting the electrostatic interaction network. From POPS titration experiments monitored by a full set of one- and two-dimensional NOESY spectra, the association between the phospholipids and the PMP1 peptide has been followed. Our data reveal that the clustering of POPS molecules is promoted from a stabilized framework obtained by coupling the PMP1 helix groove to a POPS sn-2 chain. To our knowledge, the NOE-based titration plots displayed in this report constitute the first NMR data that directly distinguish the role of the sn-1 and sn-2 acyl chains in a lipid-protein interaction. The results are discussed while taking into account our accurate knowledge of the yeast plasma membrane composition and its ability to form functional lipid rafts.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-00166870
Contributor : Françoise Baleux <>
Submitted on : Friday, August 10, 2007 - 4:34:56 PM
Last modification on : Monday, January 13, 2020 - 5:08:05 PM

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Florence Mousson, Yves-Marie Coïc, Françoise Baleux, Veronica Beswick, Alain Sanson, et al.. Deciphering the role of individual acyl chains in the interaction network between phosphatidylserines and a single-spanning membrane protein.. Biochemistry, American Chemical Society, 2002, 41 (46), pp.13611-6. ⟨10.1021/bi026274b⟩. ⟨pasteur-00166870⟩

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