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Poliovirus transcytosis through M-like cells.

Abstract : During the digestive-tract phase of infection, poliovirus (PV) is found in the oropharynx and the intestine. It has been proposed that PV enters the organism by crossing M cells, which are scattered in the epithelial sheet covering lymphoid follicles of Peyer's patches. However, PV translocation through M cells has never been demonstrated. A model of M-like cells has been previously developed using monolayers of polarized Caco-2 enterocytes cocultured with lymphocytes isolated from Peyer's patches. In this model, lymphoepithelial interactions trigger the appearance of epithelial cells having morphological and functional characteristics of M cells. We have demonstrated efficient, temperature-dependent PV transcytosis in Caco-2 cell monolayers containing M-like cells. This experimental evidence is consistent with M cells serving as gateways allowing PV access to the basal face of enterocytes, the underlying immune follicle cells, and PV transport toward mesenteric lymph nodes.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-00166731
Contributor : Hélène Ribierre <>
Submitted on : Friday, August 10, 2007 - 9:43:40 AM
Last modification on : Monday, January 13, 2020 - 5:08:10 PM

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Laurent Ouzilou, Elise Caliot, Isabelle Pelletier, Marie-Christine Prévost, Eric Pringault, et al.. Poliovirus transcytosis through M-like cells.. Journal of General Virology, Microbiology Society, 2002, 83 (Pt 9), pp.2177-82. ⟨10.1099/0022-1317-83-9-2177⟩. ⟨pasteur-00166731⟩

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