Skip to Main content Skip to Navigation
New interface
Journal articles

GPI-anchored proteins are directly targeted to the apical surface in fully polarized MDCK cells.

Abstract : The polarity of epithelial cells is dependent on their ability to target proteins and lipids in a directional fashion. The trans-Golgi network, the endosomal compartment, and the plasma membrane act as sorting stations for proteins and lipids. The site of intracellular sorting and pathways used for the apical delivery of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) are largely unclear. Using biochemical assays and confocal and video microscopy in living cells, we show that newly synthesized GPI-APs are directly delivered to the apical surface of fully polarized Madin-Darby canine kidney cells. Impairment of basolateral membrane fusion by treatment with tannic acid does not affect the direct apical delivery of GPI-APs, but it does affect the organization of tight junctions and the integrity of the monolayer. Our data clearly demonstrate that GPI-APs are directly sorted to the apical surface without passing through the basolateral membrane. They also reinforce the hypothesis that apical sorting of GPI-APs occurs intracellularly before arrival at the plasma membrane.
Document type :
Journal articles
Complete list of metadata

Cited literature [46 references]  Display  Hide  Download
Contributor : Christiane Goisnard Connect in order to contact the contributor
Submitted on : Wednesday, August 8, 2007 - 12:54:36 PM
Last modification on : Wednesday, November 30, 2022 - 4:14:07 PM
Long-term archiving on: : Thursday, April 8, 2010 - 9:10:55 PM


Publisher files allowed on an open archive




Simona Paladino, Thomas Pocard, Maria Agata Catino, Chiara Zurzolo. GPI-anchored proteins are directly targeted to the apical surface in fully polarized MDCK cells.. Journal of Cell Biology, 2006, 172 (7), pp.1023-34. ⟨10.1083/jcb.200507116⟩. ⟨pasteur-00166614⟩



Record views


Files downloads