Analysis of detergent-resistant membranes associated with apical and basolateral GPI-anchored proteins in polarized epithelial cells.

Simona Tivodar (1) , Simona Paladino (1, 2, 3) , Rudolf Pillich (1) , Alessandro Prinetti (4) , Vanna Chigorno (4) , Gerrit van Meer (5) , Sandro Sonnino (4) , Chiara Zurzolo (1, 3)

1 Dipartimento di Biologia e Patologia Cellulare e Moleculare
2 CEINGE Biotecnologie Avanzate s.c.a.r.l.
3 Trafic membranaire et Pathogénèse
4 Center of Excellence on Neurodegenerative Diseases
5 Department of Membrane Enzymology

Detergent-resistant membranes (DRMs) represent specialized membrane domains resistant to detergent extraction, which may serve to segregate proteins in a specific environment in order to improve their function. Segregation of glycosylphosphatidylinositol-anchored proteins (GPI-APs) in DRMs has been shown to be involved in their sorting to the apical membrane in polarized epithelial cells. Nonetheless, we have shown that both apical and basolateral GPI-APs associate with DRMs. In this report we investigated the lipid composition of DRMs associated with an apical and a basolateral GPI-AP. We found that apical and basolateral DRMs contain the same lipid species although in different ratios. This specific lipid ratio is maintained after mixing the cells before lysis indicating that DRMs maintain their identity after Triton extraction.

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Molecular biology

Simona Tivodar, Simona Paladino, Rudolf Pillich, Alessandro Prinetti, Vanna Chigorno, et al.. Analysis of detergent-resistant membranes associated with apical and basolateral GPI-anchored proteins in polarized epithelial cells.. FEBS Letters, 2006, 580 (24), pp.5705-12. ⟨10.1016/j.febslet.2006.09.022⟩. ⟨pasteur-00166613⟩