Synthesis and incorporation into DNA fragments of the artificial nucleobase, 2-amino-8-oxopurine. - Institut Pasteur Access content directly
Journal Articles Bioorganic and Medicinal Chemistry Letters Year : 2005

Synthesis and incorporation into DNA fragments of the artificial nucleobase, 2-amino-8-oxopurine.

Abstract

The nucleoside 2-amino-9-(2-deoxy-beta-d-ribofuranosyl)-7,8-dihydro-8-oxo-purine (dJ) was obtained in eight steps from 2'-deoxyguanosine. The appropriate protected phosphoramidite was synthesized and incorporated into DNA oligonucleotides. The thermal stability of heteroduplexes containing 2-amino-8-oxopurine (J) was investigated by UV-thermal denaturation experiments. The results obtained can be interpreted by the base pairing schemes involving the two edges of dJ depending on the anti and syn orientations.

Dates and versions

pasteur-00166211 , version 1 (01-08-2007)

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Claudio Cadena-Amaro, Muriel Delepierre, Sylvie Pochet. Synthesis and incorporation into DNA fragments of the artificial nucleobase, 2-amino-8-oxopurine.. Bioorganic and Medicinal Chemistry Letters, 2005, 15 (4), pp.1069-73. ⟨10.1016/j.bmcl.2004.12.021⟩. ⟨pasteur-00166211⟩

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