Skip to Main content Skip to Navigation
Journal articles

Synthesis and recognition by DNA polymerases of a reactive nucleoside for DNA diversification.

Abstract : The synthesis of 1-(2-deoxy-beta-D-erythro-pentofuranosyl)imidazole-4-hydrazide having the features of an ambigous base is reported. The recognition of the analogue by DNA polymerases as an incoming triphosphate as well as a template base was investigated. The mutagenic properties was evaluated by PCR. The potential of this new monomer for DNA diversification is illustrated by the reactivity of the nucleobase towards various aldehydes.
Document type :
Journal articles
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-00166203
Contributor : Sylvie Pochet <>
Submitted on : Wednesday, August 1, 2007 - 6:53:01 PM
Last modification on : Monday, January 13, 2020 - 5:08:05 PM

Identifiers

Collections

Citation

Sylvie Pochet, Laurence Dugué, Heike Strobel. Synthesis and recognition by DNA polymerases of a reactive nucleoside for DNA diversification.. Nucleosides, Nucleotides and Nucleic Acids, Taylor & Francis, 2003, 22 (5-8), pp.1665-7. ⟨10.1081/NCN-120023108⟩. ⟨pasteur-00166203⟩

Share

Metrics

Record views

160