Synthesis and recognition by DNA polymerases of a reactive nucleoside for DNA diversification.

Sylvie Pochet (1) , Laurence Dugué (1) , Heike Strobel (1)

1 Chimie Organique

The synthesis of 1-(2-deoxy-beta-D-erythro-pentofuranosyl)imidazole-4-hydrazide having the features of an ambigous base is reported. The recognition of the analogue by DNA polymerases as an incoming triphosphate as well as a template base was investigated. The mutagenic properties was evaluated by PCR. The potential of this new monomer for DNA diversification is illustrated by the reactivity of the nucleobase towards various aldehydes.

Domaines: Sciences du Vivant [q-bio]

Sylvie Pochet, Laurence Dugué, Heike Strobel. Synthesis and recognition by DNA polymerases of a reactive nucleoside for DNA diversification.. Nucleosides, Nucleotides and Nucleic Acids, 2003, 22 (5-8), pp.1665-7. ⟨10.1081/NCN-120023108⟩. ⟨pasteur-00166203⟩