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Nicotinic receptors regulate B lymphocyte activation and immune response.

Abstract : The presence of nicotinic acetylcholine receptors (nicotinic receptors) composed of either alpha7 or alpha4 and beta2 subunits is revealed in B lymphocytes by means of radioligand binding assay and Cell ELISA. Mouse B lymphocytes contained 12,200+/-3200 of epibatidine-binding sites and 3130+/-750 of alpha-Bungarotoxin-binding sites per cell. Mice lacking nicotinic receptor subunits alpha4, beta2 or alpha7 had less serum IgG and IgG-producing cells in the spleen, but showed stronger immune response to both protein antigen in vivo and CD40-specific antibody in vitro than wild-type mice. Anti-CD40-stimulated proliferation of B lymphocytes from beta2 knockout, but not wild-type mice was inhibited with nicotine. Our results indicate that signalling through nicotinic receptors affects both the pre-immune state and activation of B lymphocytes in the immune response, possibly via CD40-dependent pathway. This could contribute to immune depression found in tobacco smokers.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-00162558
Contributor : Yolande Meunier <>
Submitted on : Friday, July 13, 2007 - 4:52:32 PM
Last modification on : Wednesday, October 14, 2020 - 4:10:06 AM

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Marina Skok, Régis Grailhe, Jean-Pierre Changeux. Nicotinic receptors regulate B lymphocyte activation and immune response.. European Journal of Pharmacology, Elsevier, 2005, 517 (3), pp.246-51. ⟨10.1016/j.ejphar.2005.05.011⟩. ⟨pasteur-00162558⟩

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