Perinatal exposure to nicotine causes deficits associated with a loss of nicotinic receptor function.

Gary Cohen (1, 2) , Jean-Christophe Roux (1) , Régis Grailhe (3) , Girvan Malcolm (2) , Jean-Pierre Changeux (3) , Hugo Lagercrantz (1)

1 Departments of Woman & Child Health
2 Neonatal Medicine
3 RC - Récepteurs et Cognition

We investigated the role played by beta2-containing neuronal nicotinic receptors [nicotinic acetylcholine receptors (nAChRs)] in mediating nicotine's side effects in the fetus and newborn. Pregnant WT and mutant mice lacking the beta2 nAChR subunit were implanted with osmotic minipumps that delivered either water or a controlled dose of nicotine. Subsequently, we compared the development of the sympathoadrenal system and breathing and arousal reflexes of offspring shortly after birth, a period of increased vulnerability to nicotine exposure. Newborn WT pups exposed to nicotine exhibited all of the deficits associated with maternal tobacco and nicotine use, and linked to poor neonatal outcome: growth restriction, unstable breathing, and impaired arousal and catecholamine biosynthesis. Remarkably similar deficits were detected in pups lacking beta2-containing nAChRs. Loss-of-function of these nAChRs consequently reproduces with astonishing fidelity many of the abnormalities caused by perinatal nicotine exposure. We propose that the underlying mechanisms of nicotine's detrimental side effects on a range of crucial defensive reflexes involve loss of function of nAChR subtypes, possibly via activity-dependent desensitization.

Domaines: Neurobiologie

Gary Cohen, Jean-Christophe Roux, Régis Grailhe, Girvan Malcolm, Jean-Pierre Changeux, et al.. Perinatal exposure to nicotine causes deficits associated with a loss of nicotinic receptor function.. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102 (10), pp.3817-21. ⟨10.1073/pnas.0409782102⟩. ⟨pasteur-00162548⟩