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Live imaging of neural structure and function by fibred fluorescence microscopy.

Abstract : Only a few methods permit researchers to study selected regions of the central and peripheral nervous systems with a spatial and time resolution sufficient to image the function of neural structures. Usually, these methods cannot analyse deep-brain regions and a high-resolution method, which could repeatedly probe dynamic processes in any region of the central and peripheral nervous systems, is much needed. Here, we show that fibred fluorescence microscopy-which uses a small-diameter fibre-optic probe to provide real-time images-has the spatial resolution to image various neural structures in the living animal, the consistency needed for a sequential, quantitative evaluation of axonal degeneration/regeneration of a peripheral nerve, and the sensitivity to detect calcium transients on a sub-second timescale. These unique features should prove useful in many physiological studies requiring the in situ functional imaging of tissues in a living animal.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-00161379
Contributor : Yolande Meunier <>
Submitted on : Tuesday, July 10, 2007 - 3:34:09 PM
Last modification on : Wednesday, September 23, 2020 - 4:32:58 AM

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Pierre Vincent, Uwe Maskos, Igor Charvet, Laurence Bourgeais, Luc Stoppini, et al.. Live imaging of neural structure and function by fibred fluorescence microscopy.. EMBO Reports, EMBO Press, 2006, 7 (11), pp.1154-61. ⟨10.1038/sj.embor.7400801⟩. ⟨pasteur-00161379⟩

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