Reduced apoptosis in human intestinal cells cured of persistent poliovirus infection.

Karine Labadie (1, 2) , Aure Saulnier (1, 2) , Sandra Martin-Latil (1, 2) , Florence Colbère-Garapin (1, 2)

1 Virus Entérotropes et Stratégies Antivirales
2 BVE - Biologie des Virus entériques

Cells cured of persistent virus infection can be used to investigate cellular pathways of resistance to viral cytopathic effects. Persistent poliovirus (PV) infections were established in human intestinal Caco-2 cells, and spontaneously cured cell cultures were obtained. Two cell clones, cl6 and b13, cured of type 3 PV mutant infection and their parental Caco-2 cells were compared for susceptibility to PV infection, PV receptor CD155 expression, capacity to differentiate into polarized enterocytes, and PV-, staurosporine-, and actinomycin D-induced apoptosis. Our results strongly suggest that cells that are partially resistant to apoptosis can be selected during persistent virus infection.

Domains: Life Sciences [q-bio]; Human health and pathology; Infectious diseases

Karine Labadie, Aure Saulnier, Sandra Martin-Latil, Florence Colbère-Garapin. Reduced apoptosis in human intestinal cells cured of persistent poliovirus infection.. Journal of Virology, 2007, 81 (6), pp.3033-6. ⟨10.1128/JVI.01960-06⟩. ⟨pasteur-00146511⟩