Skip to Main content Skip to Navigation
Journal articles

Activation-induced polarized recycling targets T cell antigen receptors to the immunological synapse; involvement of SNARE complexes.

Abstract : The mechanism by which T cell antigen receptors (TCR) accumulate at the immunological synapse has not been fully elucidated. Since TCRs are continuously internalized and recycled back to the cell surface, we investigated the role of polarized recycling in TCR targeting to the immunological synapse. We show here that the recycling endosomal compartment of T cells encountering activatory antigen-presenting cells (APCs) polarizes towards the T cell-APC contact site. Moreover, TCRs in transit through recycling endosomes are targeted to the immunological synapse. Inhibition of T cell polarity, constitutive TCR endocytosis, or recycling reduces TCR accumulation at the immunological synapse. Conversely, increasing the amount of TCRs in recycling endosomes before synapse formation enhanced their accumulation. Finally, we show that exocytic t-SNAREs from T cells cluster at the APC contact site and that tetanus toxin inhibits TCR accumulation at the immunological synapse, indicating that vesicle fusion mediated by SNARE complexes is involved in TCR targeting to the immunological synapse.
Document type :
Journal articles
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-00137478
Contributor : Cécile Roux <>
Submitted on : Tuesday, March 20, 2007 - 12:57:26 PM
Last modification on : Wednesday, October 14, 2020 - 4:06:47 AM

Identifiers

Collections

Citation

Vincent Das, Béatrice Nal, Annick Dujeancourt, Maria-Isabel Thoulouze, Thierry Galli, et al.. Activation-induced polarized recycling targets T cell antigen receptors to the immunological synapse; involvement of SNARE complexes.. Immunity, Elsevier, 2004, 20 (5), pp.577-88. ⟨10.1016/S1074-7613(04)00106-2⟩. ⟨pasteur-00137478⟩

Share

Metrics

Record views

168