Skip to Main content Skip to Navigation
Journal articles

Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth

Abstract : Mitotic spindle function depends on the precise regulation of microtubule dynamics and microtubule sliding. Throughout mitosis, both processes have to be orchestrated to establish and maintain spindle stability. We show that during anaphase B spindle elongation in S. pombe , the sliding motor Klp9 (kinesin-6) also promotes microtubule growth in vivo. In vitro , Klp9 can enhance and dampen microtubule growth, depending on the tubulin concentration. This indicates that the motor is able to promote and block tubulin subunit incorporation into the microtubule lattice in order to set a well-defined microtubule growth velocity. Moreover, Klp9 recruitment to spindle microtubules is dependent on its dephosphorylation mediated by XMAP215/Dis1, a microtubule polymerase, creating a link between the regulation of spindle length and spindle elongation velocity. Collectively, we unravel the mechanism of anaphase B, from Klp9 recruitment to the motors dual-function in regulating microtubule sliding and microtubule growth, allowing an inherent coordination of both processes.
Keywords : S. pombe cell biology
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03258505
Contributor : Jacqueline Martin-Laffon <>
Submitted on : Monday, June 21, 2021 - 4:43:12 PM
Last modification on : Tuesday, July 13, 2021 - 3:28:10 AM
Long-term archiving on: : Wednesday, September 22, 2021 - 6:57:55 PM

File

elife-67489-v2.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Lara Katharina Krüger, Matthieu Gélin, Liang Ji, Carlos Kikuti, Anne Houdusse, et al.. Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth. eLife, eLife Sciences Publication, 2021, 10, ⟨10.7554/eLife.67489⟩. ⟨hal-03258505⟩

Share

Metrics

Record views

55

Files downloads

48