Antimicrobial Resistance in Wildlife in Guadeloupe (French West Indies): Distribution of a Single blaCTX–M–1/IncI1/ST3 Plasmid Among Humans and Wild Animals

Stéphanie Guyomard-Rabenirina (1) , Yann Reynaud (1) , Matthieu Pot (1) , Emmanuel Albina (2, 3) , David Couvin (1) , Célia Ducat (1) , Gaelle Gruel (1) , Séverine Ferdinand (1) , Pierre Legreneur (4) , Simon Le Hello (5, 6) , Edith Malpote (7) , Syndia Sadikalay (1) , Antoine Talarmin (1) , Sébastien Breurec (1, 8, 9)

1 TReD-Path - Unité Transmission, Réservoir et Diversité des Pathogènes [Pasteur Guadeloupe, France]
2 UMR ASTRE - Animal, Santé, Territoires, Risques et Ecosystèmes
3 Cirad-BIOS - Département Systèmes Biologiques
4 LIBM - Laboratoire Interuniversitaire de Biologie de la Motricité
5 UNICAEN - Université de Caen Normandie
6 BPE - Bactéries pathogènes entériques
7 CHU Pointe-à-Pitre/Abymes [Guadeloupe]
8 UA UFR SM - Université des Antilles - UFR des sciences médicales Hyacinthe Bastaraud
9 Clinical Investigation and Epidemiology Center, INSERM 802, Guadeloupe

Limited data are available on the contribution of wildlife to the spread of antibacterial resistance. We determined the prevalence of resistance to antibiotics inEscherichia coliisolates collected from wild animals in 2013 and 2014 and the genetic basis for resistance to third-generation cephalosporin in Guadeloupe. We recovered 52 antibiotic-resistant (AR)E. colistrains from 48 of the 884 (5.4%) wild animals tested (46 iguanas, 181 birds, 289 anoles, and 368 rodents at 163 sampling sites). Rodents had higher rates of carriage (n= 38, 10.3%) than reptiles and birds (2.4% and 1.1%, respectively,p< 0.001). A significant association (p< 0.001) was found between the degree of anthropization and the frequency of ARE. colicarriage for all species. The carriage rate of ciprofloxacin- and cefotaxime-resistant isolates was 0.7% (6/884) and 1.5% (13/884), respectively. Most (65.4%) ARE. coliwere multi-drug resistant, and the prevalence of extended-spectrum beta-lactamase (ESBL)-producingE. coliwas low (n= 7, 0.8%) in all species. Eight ESBL-producingE. coliwere recovered, two genetically unrelated isolates being found in one bird. These isolates and 20 human invasive ESBLE. coliisolates collected in Guadeloupe during the same period were investigated by whole genome sequencing.bla(CTX-M-1)was the only ESBL gene shared by three animal classes (humans,n= 2; birds,n= 2; rodents,n= 2). Thebla(CTX-M-1)gene and most of the antimicrobial resistance genes were present in a large conjugative IncI1 plasmid that was highly similar (>99% nucleotide identity) to ESBL-carrying plasmids found in several countries in Europe and in Australia. Although the prevalence of ESBL-producingE. coliisolates was very low in wild animals, it is of concern that the well-conserved IncI1 plasmid-carryingbla(CTX-M-1)is widespread and occurs in variousE. colistrains from animals and humans.

Mots clés: Escherichia coli; wild animals; antimicrobial resistance; extended-spectrum beta-lactamase; plasmid; Escherichia coli

Domaines: Sciences du Vivant [q-bio]

Stéphanie Guyomard-Rabenirina, Yann Reynaud, Matthieu Pot, Emmanuel Albina, David Couvin, et al.. Antimicrobial Resistance in Wildlife in Guadeloupe (French West Indies): Distribution of a Single blaCTX–M–1/IncI1/ST3 Plasmid Among Humans and Wild Animals. Frontiers in Microbiology, 2020, 11, ⟨10.3389/fmicb.2020.01524⟩. ⟨hal-02928330⟩