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ComFC mediates transport and handling of single-stranded DNA during natural transformation

Abstract : Abstract The ComFC protein is essential for natural transformation, a process that plays a major role in the spread of antibiotic resistance genes and virulence factors across bacteria. However, its role remains largely unknown. Here, we show that Helicobacter pylori ComFC is involved in DNA transport through the cell membrane, and is required for the handling of the single-stranded DNA once it is delivered into the cytoplasm. The crystal structure of ComFC includes a zinc-finger motif and a putative phosphoribosyl transferase domain, both necessary for the protein’s in vivo activity. Furthermore, we show that ComFC is a membrane-associated protein with affinity for single-stranded DNA. Our results suggest that ComFC provides the link between the transport of the transforming DNA into the cytoplasm and its handling by the recombination machinery.
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https://hal.archives-ouvertes.fr/hal-03650421
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Submitted on : Thursday, May 12, 2022 - 12:41:55 PM
Last modification on : Saturday, May 21, 2022 - 3:20:41 AM

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Prashant Damke, Louisa Celma, Sumedha Kondekar, Anne Marie Di Guilmi, Stéphanie Marsin, et al.. ComFC mediates transport and handling of single-stranded DNA during natural transformation. Nature Communications, Nature Publishing Group, 2022, 13 (1), pp.1961. ⟨10.1038/s41467-022-29494-z⟩. ⟨hal-03650421⟩

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