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Longitudinal high-resolution imaging through a flexible intravital imaging window

Abstract : Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, sutureless design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening previously unattainable avenues for in vivo imaging of soft and fragile tissues.
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https://hal.sorbonne-universite.fr/hal-03282426
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Submitted on : Friday, July 16, 2021 - 11:31:08 AM
Last modification on : Thursday, September 16, 2021 - 9:17:44 AM

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Guillaume Jacquemin, Maria Benavente-Diaz, Samir Djaber, Aurélien Bore, Virginie Dangles-Marie, et al.. Longitudinal high-resolution imaging through a flexible intravital imaging window. Science Advances , American Association for the Advancement of Science (AAAS), 2021, 7 (25), pp.eabg7663. ⟨10.1126/sciadv.abg7663⟩. ⟨hal-03282426⟩

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