| PMID : |
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 (22539299) |
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| titre : |
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Innate NKTγδ and NKTαβ cells exert similar functions and compete for a thymic niche. |
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| auteur(s) : |
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Pablo Pereira ( ) 1, Laurent Boucontet1 |
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| laboratoire : |
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| 1 : |
Lymphopoïèse |
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| Institut Pasteur de Paris – INSERM : U668 |
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| 25-28, rue du Docteur Roux 75724 Paris cedex 15 |
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| France |
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| résumé : |
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The transcriptional regulator promyelocytic leukemia zinc finger (PLZF) is highly expressed during the differentiation of natural killer T (NKT) cells and is essential for the acquisition of their effector/memory innate-like phenotype. Staining with anti-PLZF and anti-NK1.1 Abs allows the definition of two subsets of NKTαβ and NKTγδ thymocytes that differ phenotypically and functionally: a PLZF(+) NK1.1(-) subset composed of mostly quiescent cells that secrete more IL-4 than IFN-γ upon activation and a PLZF(+/-) NK1.1(+) subset that expresses CD127, NK1.1, and other NK-cell markers, secrete more IFN-γ than IL-4 upon activation and contains a sizable fraction of dividing cells. The size of the NK1.1(+) population is very tightly regulated and NK1.1(+) αβ and γδ thymocytes compete for a thymic niche. Furthermore, the relative representation of the PLZF(+) and NK1.1(+) subsets varies in a strain-specific manner with C57BL/6 (B6) mice containing more NK1.1(+) cells and (B6 × DBA/2)F1 (B6D2F1) mice more PLZF(+) cells. Consequently, activation of NKT cells in vivo is expected to result in higher levels of IL-4 secreted in B6D2F1 mice than in B6 mice. Consistent with this possibility, B6D2F1 mice, when compared with B6 mice, contain more "innate" CD8(+) thymocytes, the generation of which depends on IL-4 secreted by NKT cells. |
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| domaine : |
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Sciences du Vivant/Immunologie
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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0014-2980 |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1002/eji.201142109 |
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| journal : |
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| European Journal of Immunology (Eur J Immunol) |
| Publisher |
Wiley-VCH Verlag Berlin |
| ISSN |
0014-2980 (eISSN : 1521-4141) |
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| Audience : |
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internationale |
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| date de publication : |
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05/2012 |
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| volume : |
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42 |
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| numéro : |
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5 |
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| page, identifiant, ... : |
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1272-81 |
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| contrat, financement : |
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Institutional funding from the Institut Pasteur and the Institut National de la Santé et la Recherche Medicale (INSERM) |
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