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 (18344974) |
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| titre : |
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Clathrin-independent endocytosis used by the IL-2 receptor is regulated by Rac1, Pak1 and Pak2. |
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| auteur(s) : |
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Alexandre Grassart1, Annick Dujeancourt1, Paul B. Lazarow1, Alice Dautry-Varsat1, Nathalie Sauvonnet ( ) 1 |
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| laboratoire : |
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| résumé : |
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There are several endocytic pathways, which are either dependent on or independent of clathrin. This study focuses on a poorly characterized mechanism-clathrin- and caveolae-independent endocytosis-used by the interleukin-2 receptor beta (IL-2R beta). We address the question of its regulation in comparison with the clathrin-dependent pathway. First, we show that Ras-related C3 botulinum toxin substrate 1 (Rac1) is specifically required for IL-2R beta entry, and we identify p21-activated kinases (Paks) as downstream targets. By RNA interference, we show that Pak1 and Pak2 are both necessary for IL-2R beta uptake, in contrast to the clathrin-dependent route. We observe that cortactin, a partner of actin and dynamin-two essential endocytic factors-is required for IL-2R beta uptake. Furthermore, we find that cortactin acts downstream from Paks, suggesting control of its function by these kinases. Thus, we describe a cascade composed of Rac1, Paks and cortactin specifically regulating IL-2R beta internalization. This study indicates Paks as the first specific regulators of the clathrin-independent endocytosis pathway. |
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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1469-221X |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1038/embor.2008.28 |
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| journal : |
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| EMBO reports (EMBO Rep) |
| Publisher |
Nature Publishing Group: EMBO |
| ISSN |
1469-221X (eISSN : 1469-3178) |
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| Audience : |
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internationale |
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| date de publication : |
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04/2008 |
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| volume : |
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9 |
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| numéro : |
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4 |
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| page, identifiant, ... : |
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356-62 |
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| Descripteur(s) MeSH : |
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Cell Line – Clathrin – Cortactin – Endocytosis – Humans – Microscopy – Fluorescence – RNA Interference – Receptors – Interleukin-2 – p21-Activated Kinases – rac1 GTP-Binding Protein |
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| contrat, financement : |
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Action Concertée, Programme Dynamique et Réactivité des Assemblages Biologiques Action Concertée Biologie Cellulaire, Moléculaire et Structurale. |
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