| Domaine : |
 |
Sciences du Vivant/Biologie cellulaire
|
|
PMID (identifiant
de la référence Pubmed) : |
|
 (22153078) |
|
| Titre : |
|
Sec22b regulates phagosomal maturation and antigen crosspresentation by dendritic cells. |
|
| Auteur(s) : |
|
Ignacio Cebrian1, Geraldine Visentin1, Nicolas Blanchard2, Mabel Jouve3, Alexandre Bobard4, Catarina Moita5, Jost Enninga4, Luis F Moita5, Sebastian Amigorena1, Ariel Savina1 |
|
| Laboratoire : |
|
|
|
| Résumé : |
|
Antigen (Ag) crosspresentation by dendritic cells (DCs) involves the presentation of internalized Ags on MHC class I molecules to initiate CD8+ T cell-mediated immunity in response to certain pathogens and tumor cells. Here, we identify the SNARE Sec22b as a specific regulator of Ag crosspresentation. Sec22b localizes to the ER-Golgi intermediate compartment (ERGIC) and pairs to the plasma membrane SNARE syntaxin 4, which is present in phagosomes (Phgs). Depletion of Sec22b inhibits the recruitment of ER-resident proteins to Phgs and to the vacuole containing the Toxoplasma gondii parasite. In Sec22b-deficient DCs, crosspresentation is compromised after Ag phagocytosis or endocytosis and after invasion by T. gondii. Sec22b silencing inhibited Ag export to the cytosol and increased phagosomal degradation by accelerating lysosomal recruitment. Our findings provide insight into an intracellular traffic pathway required for crosspresentation and show that Sec22b-dependent recruitment of ER proteins to Phgs critically influences phagosomal functions in DCs. |
|
Langue du texte
intégral : |
|
Anglais |
|
|
| Type de publication : |
|
Articles dans des revues avec comité de lecture |
|
| DOI : |
|
10.1016/j.cell.2011.11.021 |
|
| Journal : |
|
| Cell (Cell) |
| Publisher |
Elsevier (Cell Press) |
| ISSN |
0092-8674 |
|
|
| Audience : |
|
internationale |
|
| Date de publication : |
|
09/12/2011 |
|
| Volume : |
|
147 |
|
| Numéro : |
|
6 |
|
| Page, identifiant, ... : |
|
1355-68 |
|
|
| Référence interne : |
|
Plate-forme ImagoSeine : Microscopie électronique (Resp . Maïté Coppey) |
|
|
| Contrat, financement : |
|
Institut Curie,Institut National de la Santé et de la Recherche Médicale (INSERM), ARC (Association de la Recherche contre le Cancer, N° SFI20101201629), Ligue National de Lutte contre le Cancer (Ligue labélisée 2007-2010 and 2011-2013), European Research Council (PhagoDC n°233062), Agence National de Recherche (ANR MIME APAT), Bettencourt-Schueller Foundation (FBS), Human Frontier Science Program Organization, Curie Institute PhD Scholarship program, Fundação Luso-Americana para o Desenvolvimento and Fundação para a Ciência e a Tecnologia (PTDC/SAU-IMU/110303/2009, PTDC/SAU-MII/100780/2008, and PIC/IC/82991/2007) |
|
