| Domaine : |
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Sciences du Vivant/Microbiologie et Parasitologie
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PMID (identifiant
de la référence Pubmed) : |
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 (19064255) |
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| Titre : |
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TLR-independent type I interferon induction in response to an extracellular bacterial pathogen via intracellular recognition of its DNA. |
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| Auteur(s) : |
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Marie Charrel-Dennis1, Eicke Latz1, Kristen A. Halmen1, Patrick Trieu-Cuot2, Katherine A. Fitzgerald1, Dennis L. Kasper3, 4, Douglas T. Golenbock1 |
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| Laboratoire : |
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| Résumé : |
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Type I interferon (IFN) is an important host defense cytokine against intracellular pathogens, mainly viruses. In assessing IFN production in response to group B streptococcus (GBS), we find that IFN-beta was produced by macrophages upon stimulation with both heat-killed and live GBS. Exposure of macrophages to heat-killed GBS activated a Toll-like receptor (TLR)-dependent pathway, whereas live GBS activated a TLR/NOD/RIG-like receptor (RLR)-independent pathway. This latter pathway required bacterial phagocytosis, proteolytic bacterial degradation, and phagolysosomal membrane destruction by GBS pore-forming toxins, leading to the release of bacterial DNA into the cytosol. GBS DNA in the cytosol induced IFN-beta production via a pathway dependent on the activation of the serine-threonine kinase TBK1 and phosphorylation of the transcription factor IRF3. Thus, activation of IFN-alpha/-beta production during infection with GBS, commonly considered an extracellular pathogen, appears to result from the interaction of GBS DNA with a putative intracellular DNA sensor or receptor. |
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Langue du texte
intégral : |
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Anglais |
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| Type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1016/j.chom.2008.11.002 |
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| Journal : |
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| Cell Host & Microbe |
| Publisher |
Cell Press |
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| Audience : |
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internationale |
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| Date de publication : |
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11/12/2008 |
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| Volume : |
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4 |
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| Numéro : |
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6 |
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| Page, identifiant, ... : |
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543-54 |
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