The Siva protein is a novel intracellular ligand of the CD4 receptor that promotes HIV-1 envelope-induced apoptosis in T-lymphoid cells.

Bénédicte Py; Jérôme Bouchet; Guillaume Jacquot; Nathalie Sol-Foulon; Stéphane Basmaciogullari; Olivier Schwartz; Martine Biard-Piechaczyk; Serge Benichou

hal-00257277, version 1

The Siva protein is a novel intracellular ligand of the CD4 receptor that promotes HIV-1 envelope-induced apoptosis in T-lymphoid cells.
Py B., Bouchet J., Jacquot G., Sol-Foulon N., Basmaciogullari S., Schwartz O., Biard-Piechaczyk M., Benichou S.
APOPTOSIS 12, 10 (2007) 1879-92 - http://hal.archives-ouvertes.fr/hal-00257277

Domaine :

Sciences du Vivant/Biochimie, Biologie Moléculaire

PMID (identifiant
de la référence Pubmed) :

(17653867)

Titre :

The Siva protein is a novel intracellular ligand of the CD4 receptor that promotes HIV-1 envelope-induced apoptosis in T-lymphoid cells.

Auteur(s) :

Bénédicte Py¹, Jérôme Bouchet¹, Guillaume Jacquot¹, Nathalie Sol-Foulon², Stéphane Basmaciogullari¹, Olivier Schwartz², Martine Biard-Piechaczyk³, Serge Benichou¹

Laboratoire :

1 :	IC - Institut Cochin

http://www.cochin.inserm.fr

CNRS : UMR8104 – INSERM : U567 – Université Paris V - Paris Descartes

Direction,services Communs,plateformes Bâtiment MECHAIN 22 rue Méchain 75014 PARIS

France

2 :	Virus et Immunité

CNRS : URA1930 – Institut Pasteur de Paris

28, rue du docteur Roux F-75724 Paris cedex 15

France

3 :	CPBS - Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé

Université Montpellier I – CNRS : UMR5236 – Université Montpellier II - Sciences et techniques

Institut de Biologie CS 69033 4 boulevard Henri IV 34965 MONTPELLIER Cedex 2

France

Résumé :

In addition to its positive signaling function in the antigen presentation process, CD4 acts as the primary receptor for HIV-1. Contact between CD4 and the viral envelope leads to virus entry, but can also trigger apoptosis of uninfected CD4+ T-cells through a mechanism that is poorly understood. We show that Siva-1, a death domain-containing proapoptotic protein, associates with the cytoplasmic domain of CD4. This interaction is mediated by the cysteine-rich region found in the C-terminal part of the Siva-1 protein. Expression of Siva-1 specifically increases the susceptibility of both T-cell lines and unstimulated human primary CD4+ T-lymphocytes to CD4-mediated apoptosis triggered by the HIV-1 envelope, and results in activation of a caspase-dependent mitochondrial pathway. The same susceptibility is observed in T-cells expressing a truncated form of CD4 that is able to recruit Siva-1 but fails to associate with p56Lck, indicating that Siva-1 participates in a pathway independent of the p56Lck kinase activity. Altogether, these results suggest that Siva-1 might participate in the CD4-initiated signaling apoptotic pathway induced by the HIV-1 envelope in T-lymphoid cells.

Langue du texte
intégral :

Anglais

Type de publication :

Articles dans des revues avec comité de lecture

DOI :

10.1007/s10495-007-0106-4

Journal :

Apoptosis (Apoptosis)
Publisher	Springer Verlag (Germany)
ISSN	1360-8185 (eISSN : 1573-675X)

Audience :

non spécifiée

Date de publication :

10/2007

Volume :

Numéro :

Page, identifiant, ... :

1879-92


hal-00257277, version 1
http://hal.archives-ouvertes.fr/hal-00257277
oai:hal.archives-ouvertes.fr:hal-00257277
Contributeur : Nathalie Pasqualini <>
Soumis le : Lundi 18 Février 2008, 22:53:19
Dernière modification le : Mercredi 27 Février 2008, 11:41:08