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Immunization route dictates cross-priming efficiency and impacts the optimal timing of adjuvant delivery.
Bouvier I., Jusforgues-Saklani H., Lim A., Lemaître F., Lemercier B., Auriau C., Nicola M.-A., Leroy S., Law H. K., Bandeira A. et al
Front Immunol 2 (2011) 71 - http://hal-pasteur.archives-ouvertes.fr/pasteur-00762114
(22566860)
Immunization route dictates cross-priming efficiency and impacts the optimal timing of adjuvant delivery.
Isabelle Bouvier1, Hélène Jusforgues-Saklani1, Annick Lim2, Fabrice Lemaître3, Brigitte Lemercier2, Charlotte Auriau1, Marie-Anne Nicola4, Sandrine Leroy5, Helen K Law6, Antonio Bandeira7, James J Moon8, Philippe Bousso3, Matthew L Albert () 1
1 :  Immunobiologie des Cellules Dendritiques
INSERM : U818 – Institut Pasteur de Paris
25-28 rue du Docteur Roux, F-75724 Paris Cedex 15
France
2 :  Régulation Immunitaire et Vaccinologie
INSERM : U1041 – Institut Pasteur de Paris
28 rue du Docteur Roux 75724 Paris Cedex 15
France
3 :  Dynamique des Réponses Immunes
INSERM : U668 – Institut Pasteur de Paris
25-28 rue du Docteur Roux F-75724 Paris Cedex 15
France
4 :  PFID - Imagerie Dynamique (Plate-Forme)
Institut Pasteur de Paris
25-28 rue du Docteur Roux, F-75724 Paris Cedex 15
France
5 :  Epidémiologie des Maladies Emergentes
Institut Pasteur de Paris
25-28 rue du Docteur Roux F-75724 Paris Cedex 15
France
6 :  CIH - Centre d'immunologie humaine
INSERM – Institut Pasteur de Paris
28, rue du Docteur Roux 75724 Paris cedex 15
France
7 :  Biologie des Populations Lymphocytaires
CNRS : URA1961 – Institut Pasteur de Paris
28 rue du Docteur Roux F-75724 Paris Cedex 15
France
8 :  Department of Microbiology and Center for Immunology
University of Minnesota Medical School
Minneapolis
États-Unis
Delivery of cell-associated antigen represents an important strategy for vaccination. While many experimental models have been developed in order to define the critical parameters for efficient cross-priming, few have utilized quantitative methods that permit the study of the endogenous repertoire. Comparing different strategies of immunization, we report that local delivery of cell-associated antigen results in delayed T cell cross-priming due to the increased time required for antigen capture and presentation. In comparison, delivery of disseminated antigen resulted in rapid T cell priming. Surprisingly, local injection of cell-associated antigen, while slower, resulted in the differentiation of a more robust, polyfunctional, effector response. We also evaluated the combination of cell-associated antigen with poly I:C delivery and observed an immunization route-specific effect regarding the optimal timing of innate immune stimulation. These studies highlight the importance of considering the timing and persistence of antigen presentation, and suggest that intradermal injection with delayed adjuvant delivery is the optimal strategy for achieving CD8⁺ T cell cross-priming.
Sciences du Vivant/Immunologie
Anglais
1664-3224

Articles dans des revues avec comité de lecture
10.3389/fimmu.2011.00071
Front Immunol
internationale
2011
08/12/2011
2
71

This work was supported by La Ligue contre le cancer and the EURYIscheme and by the Association pour la Recherche sur le Cancer (projectDOC20110603227).
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