A first assessment of the genetic diversity of Mycobacterium tuberculosis complex in Cambodia. - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue BMC Infectious Diseases Année : 2011

A first assessment of the genetic diversity of Mycobacterium tuberculosis complex in Cambodia.

Résumé

BACKGROUND: Cambodia is among the 22 high-burden TB countries, and has one of the highest rates of TB in South-East Asia. This study aimed to describe the genetic diversity among clinical Mycobacterium tuberculosis complex (MTC) isolates collected in Cambodia and to relate these findings to genetic diversity data from neighboring countries. METHODS: We characterized by 24 VNTR loci genotyping and spoligotyping 105 Mycobacterium tuberculosis clinical isolates collected between 2007 and 2008 in the region of Phnom-Penh, Cambodia, enriched in multidrug-resistant (MDR) isolates (n = 33). RESULTS: Classical spoligotyping confirmed that the East-African Indian (EAI) lineage is highly prevalent in this area (60%-68% respectively in whole sample and among non-MDR isolates). Beijing lineage is also largely represented (30% in whole sample, 21% among non-MDR isolates, OR = 4.51, CI 95% [1.77, 11.51]) whereas CAS lineage was absent. The 24 loci MIRU-VNTR typing scheme distinguished 90 patterns with only 13 multi-isolates clusters covering 28 isolates. The clustering of EAI strains could be achieved with only 8 VNTR combined with spoligotyping, which could serve as a performing, easy and cheap genotyping standard for this family. Extended spoligotyping suggested relatedness of some unclassified "T1 ancestors" or "Manu" isolates with modern strains and provided finer resolution. CONCLUSIONS: The genetic diversity of MTC in Cambodia is driven by the EAI and the Beijing families. We validate the usefulness of the extended spoligotyping format in combination with 8 VNTR for EAI isolates in this region.
Fichier principal
Vignette du fichier
1471-2334-11-42.pdf (238.14 Ko) Télécharger le fichier
1471-2334-11-42-S1.PDF (80.91 Ko) Télécharger le fichier
1471-2334-11-42.xml (64.71 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Format : Autre
Format : Autre

Dates et versions

pasteur-00670614 , version 1 (15-02-2012)

Identifiants

Citer

Jian Zhang, Seiha Heng, Stéphanie Le Moullec, Guislaine Refregier, Brigitte Gicquel, et al.. A first assessment of the genetic diversity of Mycobacterium tuberculosis complex in Cambodia.. BMC Infectious Diseases, 2011, 11 (1), pp.42. ⟨10.1186/1471-2334-11-42⟩. ⟨pasteur-00670614⟩
222 Consultations
313 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More